Understanding and investigating how to design and develop a multimode microscope by exploring non-linear processes and exploiting their features to reach super-resolution. In this project, a custom-made near infrared (NIR) transition absorption microscope was build that we have been using in imaging single and multilayer graphene. This research is being done in the Nanoscopy group directed by Prof. Alberto Diaspro in Center for Human Technologies (CHT) of Istituto Italiano di Tecnologiain. The expected results of this project can be organized in three levels. The first major goal will be to investigate dynamic properties of unstained biological samples with high spatial and temporal resolution by employing Stimulated Raman Scattering (SRS) and Saturated Stimulated Raman Scattering (SSRS) methods. The second goal will be the development of a multimodal imaging technology by implementing a transducer on the custom pump-probe setup to do photoacoustic imaging in terms of label free approach. A third and final step will be dedicated to applying the realized system to the biological samples and refining the configuration to achieve the best results.